The ratio of mean fluorescence intensity values of 2C5 to isotype-matched IgG control incubated cells was used to judge binding selectivity. DNA fragmentation assay Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) was finished with a Fluorescein FragEL DNA Fragmentation Recognition Package (QIA33-1EA, Calbiochem) as indicated by supplier. advantage is Continue Reading