293T cells were transfected with the AHNAK2-pGL4.17-firefly reporter construct and a control Renilla expression vector. AHNAK2 was greatly upregulated, at least in part, by hypoxia in cancer cells. By using chromatin immune-precipitation (CHIP) and promoter-luciferase reporter assays, we identified that upregulation of AHNAK2 induced by hypoxia was hypoxia-inducible factor-1 (HIF1)-dependent. Continue Reading