To do so, we transfected p53/MDMX double knock-out MEF cells with MDMX expression vectors together with an empty vector or a plasmid encoding 14-3-3 or 14-3-3 K50E that was previously shown to be defective in MDMX-binding (29,38)

Posted on by Courtney Roberts

To do so, we transfected p53/MDMX double knock-out MEF cells with MDMX expression vectors together with an empty vector or a plasmid encoding 14-3-3 or 14-3-3 K50E that was previously shown to be defective in MDMX-binding (29,38). assays, leading to the induction of p21 in p53-null cells. Knockdown of 14-3-3 Continue Reading