After counterstaining with cresyl violet, the sections were viewed by bright-field microscopy and imaged utilizing a Dxm 1200 camera (Nikon) and Respond-1 software. Hb9-eGFP and wild-type mouse spinal-cord sections were also utilized to detect immunofluorescence of Dnmt1 and Dnmt3a specifically in electric motor neurons using Alexa-594-conjugated supplementary antibody (Invitrogen). (ALS), Continue Reading