The cells were then re-suspended in 1?mL of PBS, and two 100-L samples were taken from each conical tube to be counted with the NucleoCounter. one fully enclosed bioreactor. (DH5; Life Technologies, Ontario, Canada) then grown in Luria-Broth supplemented with 30?g/mL kanamycin as previous described;35 transfection-grade plasmid DNAs were then Continue Reading