N gene expression was determined by qPCR. puromycin and surviving cells were cultivated as a polyclonal populace termed AK-D 2.2. As there is no available IFNR-specific antibody to detect the presence of feline IFNR2 protein, we used a functional assay to evaluate the AK-D 2.2?cells for an IFN signaling-deficient phenotype. Continue Reading