Crimson vertical lines indicate substitutions of cysteines to serines

Crimson vertical lines indicate substitutions of cysteines to serines. Crimson club: the TPD1 putative indication peptide (Sp), Green club: GFP, Cyan club: the non-conserved N-terminal area, Blue club: the conserved C-terminal area, TPD1: TPD1 with no putative indication peptide, and Green series: K135GR135G mutations. (B) Traditional western blotting was utilized Continue Reading