Cell lysates were heated in 85C for 15 min to denature proteinase K. 24 h before imaging. Mistake bars stand for s.d. across specialized replicates (= 4). * 0.0001 for the tiny molecule in 20 M in comparison to DMSO. (unpaired t-test, two-tailed) (L) Consultant images from the eGFP-disruption assay. Continue Reading