Supplementary MaterialsMOVIE?S1

Supplementary MaterialsMOVIE?S1. format) were imaged every 2 s for approximately 10.5 minutes. Eight seconds of the time-lapse is presented in this video. The region of interest is circled. Download Movie S2, MOV file, 0.9 MB. Copyright ? 2020 Maldonado et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. MOVIE?S3. Four time points (0 s to 6 s) of a live-cell time-lapse movie showing colocalized wild-type Gag-CFP and RC.V8-24xMS2 RNA in the nucleus. Live QT6 cells expressing Gag-CFP, RC.V8-24xMS2, NES1-YFP-MS2-NLS, and Sun1-mCherry Atrimustine (blue/gray outline) were imaged every 2 s for approximately 10.5 minutes. Spots were generated from a colocalization channel (white spheres) created in Imaris (Bitplane) from the same region of time-lapse presented in Movie S1. Eight seconds of the time-lapse is presented in this video. The region of interest is circled. Download Movie S3, MOV file, 0.2 MB. Copyright ? 2020 Maldonado et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. MOVIE?S4. Four time points (58 s to 64 s) of a live-cell time-lapse movie showing colocalized wild-type Gag-CFP and RC.V8-24xMS2 RNA in the nucleus. Live QT6 cells expressing Gag-CFP, RC.V8-24xMS2, NES1-YFP-MS2-NLS, Atrimustine and Sun1-mCherry (blue/gray outline) were imaged every 2 s for approximately 10.5 minutes. Spots were generated from a colocalization channel (white spheres) created in Imaris (Bitplane) from the same region of time-lapse presented in Movie S2. Eight seconds of the time-lapse is presented with this video. The spot of interest can be circled having a white range. Download Film S4, MOV document, 0.3 MB. Copyright ? 2020 Maldonado et al. This article can be distributed beneath the conditions of the Innovative Commons Attribution 4.0 International permit. MOVIE?S5. Surface area making of Gag-CFP and unspliced vRNA within the nucleus. Confocal z-stacks Atrimustine of QT6 cells expressing RC.V8-Gag-CFP were captured and quantity and surface area renderings created in Imaris. Gag-CFP (reddish colored) colocalized with unspliced vRNA (smFISH-green). The cell rotates, and an orthogonal clipping aircraft transects the cell within the aircraft to show reddish colored and green Gag-RNA complexes within the interchromatin areas from the DAPI staining (blue). A surface area making was also developed from the colocalization route (white) to raised visualize Gag-unspliced RNA complexes within the interchromatin areas. Download Film S5, MOV document, 6.4 MB. Copyright ? 2020 Maldonado et al. This article can be distributed beneath the conditions of the Innovative Commons Attribution 4.0 International permit. Film?S6. Four period factors (0 s to 6 s) of the live-cell time-lapse film displaying colocalized wild-type Gag-CFP and unspliced RC.V8-Gag-CFP-24xMS2 RNA within the nucleus. Live Atrimustine QT6 cells expressing Gag-CFP within the framework of RC.V8 Gag-CFP-24xMS2 (crimson), NES1-YFP-MS2-NLS (green), untagged wild-type Gag.PR, and Sunlight1-mCherry (blue/grey format) were imaged every 2 s for about 25 minutes. The spot of interest can be circled. Download Film S6, MOV document, 1.0 MB. Copyright ? 2020 Maldonado et al. This article can be distributed beneath the Atrimustine conditions of the Innovative Commons Attribution 4.0 International permit. Film?S7. Four period factors (0 s to 6 s) of the live-cell time-lapse film of wild-type Gag-CFP and unspliced RC.V8-24xMS2 RNA within the nucleus. Live QT6 cells expressing Gag-CFP within the framework of RC.V8 Gag-CFP-24xMS2, Rabbit Polyclonal to ACTBL2 NES1-YFP-MS2-NLS, untagged wild-type Gag.PR, and Sunlight1-mCherry (blue/grey format) were imaged every 2 s for about 25 minutes. Places had been generated from a colocalization route (white spheres) developed in Imaris (Bitplane) through the same area of time-lapse shown in Film S6. Eight mere seconds of the time-lapse is presented in this video. The region of interest is circled. Download Movie S7, MOV file, 0.4 MB. Copyright ? 2020 Maldonado et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. MOVIE?S8. Surface rendering of Gag-SNAP tag JF646 and with unspliced vRNA in the nucleus of an acutely infected cell. Confocal z-stacks of a QT6 cell infected with RC.V8 for 48 h and transfected with Gag-SNAP tag JF646 was captured, and volume and surface renderings were created in Imaris. Gag-SNAP tag JF646 (red) colocalized with unspliced vRNA (smFISH-green). An orthogonal clipping plane transects the cell in the plane and shows red and green Gag-RNA complexes in the interchromatin spaces of the DAPI staining (blue). A surface rendering was also created of the colocalization channel (white) to better visualize Gag-unspliced RNA complexes in the interchromatin spaces. Download Movie S8, MOV file, 5.4 MB. Copyright ? 2020 Maldonado et al. This content is distributed under the terms of.